| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sulfotransferase family cytosolic 2B member 1;ST2B1;Sulfotransferase 2B1;2.8.2.2;Alcohol sulfotransferase;Hydroxysteroid sulfotransferase 2;SULT2B1;HSST2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SULT2B1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sulfotransferase 2B1 (gene SULT2B1) in human and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 41.3 kDa, and the supplier reports an observed band at about 48 kDa.
SULT2B1 is a cytosolic sulfotransferase that uses PAPS to sulfate 3β-hydroxysteroids. One isoform mainly sulfates cholesterol, producing cholesterol sulfate that helps regulate epidermal differentiation and skin barrier function, while another prefers pregnenolone and DHEA. SULT2B1 mutations cause a form of autosomal recessive congenital ichthyosis.
| Target | Sulfotransferase 2B1 (gene SULT2B1; UniProt O00204, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 41.3 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat testis tissue, A431 cells, HeLa cells, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat intestine tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the middle region of human SULT2B1. It differs from the mouse sequence at six residues and from the rat sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places sulfotransferase 2B1 in the cytoplasm, microsome and nucleus. Tissue expression noted by UniProt (human): Expressed in the stratum granulosum-stratum corneum junction in the skin (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
