| Field | Specification |
|---|---|
| Target | |
| Alternative names | Baculoviral IAP repeat-containing protein 5;Apoptosis inhibitor 4;Apoptosis inhibitor survivin;BIRC5;API4, IAP4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Survivin recombinant protein (Position: M1-D142). Human Survivin shares 84.3% and 83% amino acid (aa) sequence identity with mouse and rat Survivin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets baculoviral IAP repeat-containing protein 5 (gene BIRC5) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 16.4 kDa, and the supplier reports an observed band at about 16 kDa.
Survivin (BIRC5) is the smallest member of the inhibitor of apoptosis family and a subunit of the chromosomal passenger complex with Aurora B, INCENP and borealin. It is required for chromosome alignment and cytokinesis during mitosis and has been linked to apoptosis inhibition. Survivin is highly expressed in most tumors but low in most adult tissues.
| Target | Baculoviral IAP repeat-containing protein 5 (gene BIRC5; UniProt O15392, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 16.4 kDa |
| Observed MW | 16 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lymphoma tissue, mouse thymus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Survivin fragment (Met1–Asp142), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 84.3% and 83%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places baculoviral IAP repeat-containing protein 5 in the cytoplasm, nucleus, chromosome and midbody. Tissue expression noted by UniProt (human): Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lotti Roberta et al. (2022) Isolation of an “Early” Transit Amplifying Keratinocyte Population in Human Epidermis: A Role for the Low Affinity Neurotrophin Receptor CD271. Stem Cells. 10.1093/stmcls/sxac060
Ying Wang et al. (2017) Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells. Oncotarget. 10.18632/oncotarget.18366
Haolong Zhang et al. (2018) Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis. Life Sciences. 10.1016/j.lfs.2018.05.045
Du Zhen-Xian et al. (2006) Antisurvivin oligonucleotides inhibit growth and induce apoptosis in human medullary thyroid carcinoma cells. Experimental and Molecular Medicine. 10.1038/emm.2006.28
Huarui Tang et al. (2024) Identification of BIRC5 and HMMR as prognostic biomarkers for immune infiltration in prostate cancer. Translational Andrology and Urology. 10.21037/tau-24-359
