| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Polycomb protein SUZ12;Chromatin precipitated E2F target 9 protein;ChET 9 protein;Joined to JAZF1 protein;Suppressor of zeste 12 protein homolog;SUZ12;CHET9, JJAZ1, KIAA0160; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human SUZ12 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SUZ12 Rabbit Monoclonal Antibody is an antibody targeting SUZ12. Common applications include WB, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IEB-19; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 47 kDa; calculated MW: 83055 MW.
Boster Bio Anti-SUZ12 Rabbit Monoclonal Antibody catalog # M00583. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: SUZ12 — Polycomb protein SUZ12
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IEB-19; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 47 kDa; Calculated: 83055 MW
Biological background
Protein function (datasheet): Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. .
Cellular localization (datasheet): Nucleus .
Tissue details (datasheet): Overexpressed in breast and colon cancer. .
Research relevance and current trends
- Commonly studied in contexts related to Chromatin Modifying Enzymes,Chromatin Remodeling,Developmental Families,Domain Families,Epigenetics and Nuclear Signaling,Methylation,Transcription.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
