| Field | Specification |
|---|---|
| Target | |
| Alternative names | Synaptophysin;Major synaptic vesicle protein p38;SYP; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Synaptophysin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BGB-19 is a rabbit monoclonal antibody that recognizes synaptophysin (gene SYP) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 33.8 kDa, and the supplier reports an observed band at about 38 kDa.
Synaptophysin (SYP) is an abundant integral membrane glycoprotein of synaptic vesicles in neurons and neuroendocrine cells. It interacts with synaptobrevin and is involved in vesicle organization and synaptic plasticity, and it is one of the most widely used markers for synapses and neuroendocrine tissue.
| Target | Synaptophysin (gene SYP; UniProt P08247, human) |
|---|---|
| Clone | BGB-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.8 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:5000–1:10000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): mouse brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): PC-12 cells.
Immunogen
Synthetic peptide based on the sequence of human synaptophysin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places synaptophysin in the synapse. Tissue expression noted by UniProt (human): Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huimao Liu et al. (2026) Resveratrol Attenuates Neuroinflammation and Gut–Brain Axis Dysfunction Induced by Lead and Cadmium Co-Exposure by Modulating Gut Microbiota in Rats. Phytotherapy Research. 10.1002/ptr.70301
