| Field | Specification |
|---|---|
| Target | |
| Alternative names | Syncoilin; Syncoilin intermediate filament 1; Syncoilin-1; Sync; Sync1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Syncoilin/Sync recombinant protein (Position: D11-Q465). Mouse Sync shares 76.6% amino acid (aa) sequence identity with human Sync. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets syncoilin in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 53.6 kDa, and the supplier reports an observed band at about 72 kDa.
Syncoilin (SYNC) is an atypical type III intermediate filament protein expressed mainly in skeletal and cardiac muscle. It binds alpha-dystrobrevin and desmin and is thought to help transmit mechanical force between myofibrils and the muscle fiber surface, but it does not assemble into filaments on its own.
| Target | Syncoilin (gene Sync; UniProt Q9EPM5, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 53.6 kDa |
| Observed MW | 72 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat skeletal muscle tissue, rat heart tissue, rat H9C2(2–1) cells, rat L6 cells, mouse heart tissue, mouse C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): C2C12 cells.
Immunogen
Recombinant mouse Syncoilin/Sync fragment (Asp11–Gln465), expressed in E. coli. Sequence identity with the human ortholog: 76.6%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places syncoilin in the cytoplasm. Tissue expression noted by UniProt (mouse): Detected strongly in skeletal muscle and heart and weakly in lung (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
