| Field | Specification |
|---|---|
| Target | |
| Alternative names | Syndecan-1;SYND1;CD138;SDC1;SDC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Syndecan-1, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets syndecan-1 (gene SDC1) in human samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 32.5 kDa, and the supplier reports an observed band at about 50 kDa.
Syndecan-1 (SDC1, CD138) is a transmembrane proteoglycan carrying heparan sulfate and chondroitin sulfate chains. It binds growth factors and matrix proteins, links the cytoskeleton to the extracellular matrix and supports exosome formation, and CD138 is widely used as a plasma cell and epithelial marker.
| Target | Syndecan-1 (gene SDC1; UniProt P18827, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 32.5 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): HT1080 cells, MCF-7 cells, 293T cells, HepG2 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 40 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the C-terminal region of human syndecan-1. It differs from the mouse sequence at six residues and from the rat sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes syndecan-1 as secreted and also located in the membrane. Tissue expression noted by UniProt (human): Detected in placenta (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huang Jinyu et al. (2013) S100+ cells: A new neuro-immune cross-talkers in lymph organs. Scientific Reports. 10.1038/srep01114
