| Field | Specification |
|---|---|
| Target | |
| Alternative names | Polyphosphatidylinositol phosphatase SYNJ2; Synaptojanin-2; SYNJ2; KIAA0348 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SYNJ2 recombinant protein (Position: D18-K1495). Human SYNJ2 shares 83.8% amino acid (aa) sequence identity with rat SYNJ2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets polyphosphatidylinositol phosphatase SYNJ2 in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 165.5 kDa, and the supplier reports an observed band at about 200 kDa.
Synaptojanin-2 (SYNJ2) is a lipid phosphatase with an N-terminal SAC domain and a central inositol 5-phosphatase domain, together able to dephosphorylate several positions on phosphoinositides and inositol phosphates. By converting PI(4,5)P2 and related lipids, it takes part in clathrin-mediated endocytosis, and it is also studied in cancer cell invasion.
| Target | Polyphosphatidylinositol phosphatase SYNJ2 (gene SYNJ2; UniProt O15056, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 165.5 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human A549 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human ovarian cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human SYNJ2 fragment (Asp18–Lys1495), expressed in E. coli. Sequence identity with the rat ortholog: 83.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places polyphosphatidylinositol phosphatase SYNJ2 in the cytoplasm, cell membrane, cell projection and presynapse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
