| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transforming acidic coiled-coil-containing protein 1;Gastric cancer antigen Ga55;Taxin-1;TACC1;KIAA1103; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TACC1, different from the related mouse sequence by fourteen amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transforming acidic coiled-coil-containing protein 1 (gene TACC1) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 87.8 kDa, and the supplier reports an observed band at about 100 kDa.
TACC1 is a member of the transforming acidic coiled-coil family, proteins that associate with centrosomes and microtubules. It has been reported to interact with nuclear receptors and influence thyroid hormone and retinoic acid–driven transcription, and it is studied in cell division and cancer biology.
| Target | Transforming acidic coiled-coil-containing protein 1 (gene TACC1; UniProt O75410, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 87.8 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|
Samples with a confirmed band (WB): mouse testis tissue, U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human TACC1. It differs from the mouse sequence at fourteen residues.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places transforming acidic coiled-coil-containing protein 1 in the cytoplasm, nucleus and midbody. Tissue expression noted by UniProt (human): Isoform 1, isoform 3 and isoform 5 are ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
