| Field | Specification |
|---|---|
| Target | |
| Alternative names | Talin-1; TLN1; KIAA1027; TLN |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Talin 1, which shares 97.6% amino acid (aa) sequence identity with both mouse and rat Talin 1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects talin-1 (gene TLN1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 269.8 kDa, and the supplier reports an observed band at about 270 kDa.
Talin-1 (TLN1) is a large cytoskeletal protein that binds integrin cytoplasmic tails and actin. It is a key activator of integrins and a mechanosensitive link between the extracellular matrix and the actin cytoskeleton at focal adhesions. Talin-1 is studied in cell adhesion, migration, platelet function and mechanotransduction.
| Target | Talin-1 (gene TLN1; UniProt Q9Y490, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 269.8 kDa |
| Observed MW | 270 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human Jurkat cells, rat C6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, mouse small intestine tissue, human intestinal cancer tissue, human tonsil tissue, rat small intestine tissue, rat lung tissue, rat spleen tissue, mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the middle region of human talin 1. Sequence identity with the mouse and rat orthologs: 97.6%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places talin-1 in the cell projection, cytoplasm and cell surface. Tissue expression noted by UniProt (human): Expressed at low to non-detectable levels in many tissues but highly expressed in skin and pancreas with other tissues including kidney cortex, endocervix, testis, pituitary, liver, and spleen also showing robust expression. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yifan Wang et al. (2019) Talin promotes integrin activation accompanied by generation of tension in talin and an increase in osmotic pressure in neurite outgrowth. FASEB Journal. 10.1096/fj.201801949RR
Ying Song et al. (2020) Inward Tension of Talin and Integrin-related Osmotic Pressure are involved Synergetically in the Invasion and Metastasis of Non-small Cell Lung Cancer. Journal of Cancer. 10.7150/jca.45494
