Anti-TAOK1 Rabbit Monoclonal Antibody

SKU:BHA21010519
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Boster Bio
Boster Bio
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Overview
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Anti-TAOK1 antibody (Rabbit monoclonal, clone 30T29, IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes serine/threonine-protein kinase TAO1. Observed band in Western blot: about 116 kDa.
Target TAOK1
clone number 30T29
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M07063 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB
      • Application details: WB 1:500-2000
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target TAOK1
Alternative names Serine/threonine-protein kinase TAO1; Kinase from chicken homolog B; hKFC-B; MARK Kinase; MARKK; Prostate-derived sterile 20-like kinase 2; PSK-2; PSK2; Prostate-derived STE20-like kinase 2; Thousand and one amino acid protein kinase 1; TAOK1; hTAOK1; KIAA1361; MAP3K16
Gene ID 57551
UniProt # Q7L7X3
Host Rabbit
Clone 30T29
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human TAOK1
Molecular weight 116.1 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus. Cytoplasm, cytoskeleton, spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M07063
Main SKU BHA21010519
Rabbit Monoclonal

Product Overview

Clone 30T29 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase TAO1 (gene TAOK1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 116.1 kDa, and the supplier reports an observed band at about 116 kDa.

TAO kinase 1 (TAOK1) is a serine/threonine kinase of the STE20 family. It activates the p38 MAPK cascade by phosphorylating MAP2K3 and MAP2K6, contributes to the DNA damage checkpoint, and regulates microtubule stability through MARK2, so it is studied in stress signaling, apoptosis and neuronal development.

Target Serine/threonine-protein kinase TAO1 (gene TAOK1; UniProt Q7L7X3, human)
Clone 30T29
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 116.1 kDa
Observed MW 116 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): HeLa cells, RAW 2647 cells, C6 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human TAOK1.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase TAO1 in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in the testis, and to a lower extent also expressed in brain, placenta, colon and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 116.1 kDa; observed band at about 116 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human TAOK1.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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