| Field | Specification |
|---|---|
| Target | |
| Alternative names | TATA box-binding protein-like 1; TBP-like 1; 21 kDa TBP-like protein; Second TBP of unique DNA protein; STUD; TATA box-binding protein-related factor 2; TBP-related factor 2; TBP-like factor; TBP-related protein; TBPL1; TLF; TLP; TLP21; TRF2; TRP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TBPL1 Does not bind the TATA box. Has DNA-binding ability. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACOI-20 is a rabbit monoclonal antibody that recognizes TATA box-binding protein-like 1 (gene TBPL1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 20.9 kDa, and the supplier reports an observed band at about 23 kDa.
TBP-like protein 1 (TBPL1, also called TRF2) is a relative of TATA-box-binding protein that does not bind the TATA box. It supports a specialized transcription system at promoters carrying the TCT core element, which drives expression of most ribosomal protein genes.
| Target | TATA box-binding protein-like 1 (gene TBPL1; UniProt P62380, human) |
|---|---|
| Clone | ACOI-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.9 kDa |
| Observed MW | 23 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells, mouse testis.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TBPL1 Does not bind the TATA box.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TATA box-binding protein-like 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed, with highest levels in the testis and ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mishal Rabia et al. (2025) RNA-seq analysis of wild-type and mutated TBPL1 gene in breast cancer cells lines through CRISPR/Cas9 approach reveals novel molecular signatures. Scientific Reports. 10.1038/s41598-025-11634-2
Lu Tang et al. (2022) Diabetic oxidative stress-induced telomere damage aggravates periodontal bone loss in periodontitis. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2022.04.039
