Anti-TC10 Rabbit Monoclonal Antibody

SKU:BHA21009657
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
Click light‑blue chips for details
Anti-RHOQ antibody (Rabbit monoclonal, clone 21R60, IgG). Validated for Western blot and immunofluorescence. Reactivity: Human, Rat. Recognizes rho-related GTP-binding protein RhoQ. Observed band in Western blot: about 23 kDa.
Target RHOQ
clone number 21R60
Host Rabbit
Reactivity Human,Rat
Isotype IgG
Application(s) WB, ICC, IF
Options selector
Catalog no. Size Conjugation
M06900-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,ICC,IF
      Application details: WB 1:500-2000<br>ICC/IF 1:50-200<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target RHOQ
Alternative names Rho-related GTP-binding protein RhoQ; Ras-like protein TC10; Ras-like protein family member 7A; RHOQ; ARHQ; RASL7A; TC10
Gene ID 23433
UniProt # P17081
Host Rabbit
Clone 21R60
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Rat
Applications
  • Western Blot
  • Immunofluorescence
Immunogen A synthesized peptide derived from human TC10
Molecular weight 22.7 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus. Cytoplasm, cytoskeleton, spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M06900-1
Main SKU BHA21009657
Rabbit Monoclonal

Product Overview

Clone 21R60 is a rabbit monoclonal antibody that recognizes rho-related GTP-binding protein RhoQ in human and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 22.7 kDa, and the supplier reports an observed band at about 23 kDa.

RhoQ (also called TC10) is a Rho-family small GTPase found at the plasma membrane. It is known for a role in insulin-stimulated movement of the glucose transporter GLUT4 to the cell surface in fat cells, and it also affects filopodia formation, epithelial polarity and CFTR trafficking.

Target Rho-related GTP-binding protein RhoQ (gene RHOQ; UniProt P17081, human)
Clone 21R60
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 22.7 kDa
Observed MW 23 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200

Samples with a confirmed band (WB): 293T cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human TC10.

Reactivity Notes

The supplier lists reactivity with human and rat. UniProt places rho-related GTP-binding protein RhoQ in the cytoplasm and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 22.7 kDa; observed band at about 23 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human TC10.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today