| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rho-related GTP-binding protein RhoQ; Ras-like protein TC10; Ras-like protein family member 7A; RHOQ; ARHQ; RASL7A; TC10 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TC10 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21R60 is a rabbit monoclonal antibody that recognizes rho-related GTP-binding protein RhoQ in human and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 22.7 kDa, and the supplier reports an observed band at about 23 kDa.
RhoQ (also called TC10) is a Rho-family small GTPase found at the plasma membrane. It is known for a role in insulin-stimulated movement of the glucose transporter GLUT4 to the cell surface in fat cells, and it also affects filopodia formation, epithelial polarity and CFTR trafficking.
| Target | Rho-related GTP-binding protein RhoQ (gene RHOQ; UniProt P17081, human) |
|---|---|
| Clone | 21R60 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.7 kDa |
| Observed MW | 23 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TC10.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places rho-related GTP-binding protein RhoQ in the cytoplasm and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.