| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor 7-like 1;HMG box transcription factor 3;TCF-3;TCF7L1;TCF3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TCF3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CAH-20 is a rabbit monoclonal antibody that recognizes transcription factor 7-like 1 (gene TCF7L1) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 62.6 kDa, and the supplier reports an observed band at about 63 kDa.
TCF7L1 (also called TCF3) is an HMG-box transcription factor of the TCF/LEF family. It represses Wnt target genes in the absence of β-catenin and can activate them when β-catenin is present, and it is studied in embryonic and skin stem cell regulation.
| Target | Transcription factor 7-like 1 (gene TCF7L1; UniProt Q9HCS4, human) |
|---|---|
| Clone | CAH-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 62.6 kDa |
| Observed MW | 63 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TCF3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcription factor 7-like 1 in the nucleus. Tissue expression noted by UniProt (human): Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
