| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transcription factor 7-like 1;HMG box transcription factor 3;TCF-3;TCF7L1;TCF3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human TCF3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TCF3 TCF7L1 Rabbit Monoclonal Antibody is an antibody targeting TCF7L1. Common applications include WB, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: CAH-20; isotype: Rabbit IgG; reactivity: Human; observed MW: 85 kDa; calculated MW: 62631 MW.
Boster Bio Anti-TCF3 TCF7L1 Rabbit Monoclonal Antibody catalog # M05152. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: TCF7L1 — Transcription factor 7-like 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: CAH-20; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 85 kDa; Calculated: 62631 MW
Biological background
Protein function (datasheet): Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance. .
Cellular localization (datasheet): Nucleus.
Tissue details (datasheet): Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. .
Research relevance and current trends
- Commonly studied in contexts related to Chromatin Binding Proteins,DNA/RNA Binding,Domain Families,Epigenetics and Nuclear Signaling,Nuclear,Signaling Pathways,Stem Cells,Transcription,Transcription Factors.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
