| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1; TCF7; TCF1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TCF7, which shares 90.6% amino acid (aa) sequence identity with mouse TCF7. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transcription factor 7 (gene TCF7) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 41.6 kDa, and the supplier reports an observed band at about 50 kDa.
TCF7 (also called TCF-1) is an HMG-box transcription factor of the TCF/LEF family that works with β-catenin in Wnt signaling. It is essential for early T-cell development and for maintaining stem-like memory and progenitor-exhausted T cells, making it a key marker in T-cell and tumor immunology research.
| Target | Transcription factor 7 (gene TCF7; UniProt P36402, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 41.6 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human MOLT-4 cells, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human TCF7. Sequence identity with the mouse ortholog: 90.6%.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places transcription factor 7 in the nucleus. Tissue expression noted by UniProt (human): Predominantly expressed in T-cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ren Jing et al. (2021) LncRNA4930473A02Rik promotes cardiac hypertrophy by regulating TCF7 via sponging miR-135a in mice. Cell Death Discovery. 10.1038/s41420-021-00775-8
