| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Formimidoyltransferase-cyclodeaminase;Formiminotransferase-cyclodeaminase;FTCD;LCHC1;Glutamate formimidoyltransferase;2.1.2.5;Glutamate formiminotransferase;Glutamate formyltransferase;Formimidoyltetrahydrofolate cyclodeaminase;4.3.1.4;Formiminotetrahydrofolate cyclodeaminase;FTCD; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human TCP1 beta |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CCT2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22C16; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-TCP1 beta Rabbit Monoclonal Antibody catalog # M05524-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CCT2 (Formimidoyltransferase-cyclodeaminase).
- Antibody format: Monoclonal; clone 22C16; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CCT2 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Reported cellular localization context: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus . More abundantly located around the mother centriole. Tissue expression notes (as provided): Ubiquitous.
Research relevance and current trends
- Research context keywords from the source record include: Metabolic Signaling Pathways,Metabolism,Organelles,Pathways and Processes,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate CCT2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CCT2 expression by Western blot in cell or tissue lysates, Detect CCT2 in FFPE tissue sections by immunohistochemistry, Localize CCT2 by immunofluorescence/immunocytochemistry in cultured cells, Quantify CCT2-positive cells by flow cytometry in single-cell suspensions, Enrich CCT2 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 57 kDa; calculated MW: 58927 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 57 kDa
- Cellular localization (provided): Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus . More abundantly located around the mother centriole.
- Tissue details (provided): Ubiquitous.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
