| Field | Specification |
|---|---|
| Target | |
| Alternative names | TAR DNA-binding protein 43;TDP-43;TARDBP;TDP43; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TDP43 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABAB-20 is a rabbit monoclonal antibody that recognizes TAR DNA-binding protein 43 (gene TARDBP) in human, mouse, rat and zebrafish samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 44.7 kDa, and the supplier reports an observed band at about 40 kDa.
TDP-43 (TARDBP) is a nuclear RNA-binding protein with two RRM domains that binds GU-rich sequences to regulate splicing, mRNA stability and transport. Cytoplasmic aggregates of TDP-43 are a pathological hallmark of most ALS cases and of frontotemporal lobar degeneration, making it a central protein in neurodegeneration research.
| Target | TAR DNA-binding protein 43 (gene TARDBP; UniProt Q13148, human) |
|---|---|
| Clone | ABAB-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat, Zebrafish |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.7 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human SH-SY5Y cells, human HeLa cells, human Jurkat cells, human 293T cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human lung cancer tissue, human esophageal cancer tissue, human rectal cancer tissue, human ovarian cancer tissue, human tonsil tissue, human liver cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human TDP43.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and zebrafish. UniProt places TAR DNA-binding protein 43 in the nucleus, cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
June Bai et al. (2025) Mitochondrial Genome-Encoded lncND5 Regulates Mitophagy in Hypoxic Pulmonary Artery Smooth Muscle Cell. FASEB Journal. 10.1096/fj.202500389R
YuanJing Zhang et al. (2019) MiRNA-27a decreases ultraviolet B irradiation-induced cell damage. Journal of Cellular Biochemistry. 10.1002/jcb.29337
