| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcriptional enhancer factor TEF-1; NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13; TEAD1; TCF13; TEF1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TEAD1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24T42 is a rabbit monoclonal antibody that recognizes transcriptional enhancer factor TEF-1 (gene TEAD1) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 47.9 kDa, and the supplier reports an observed band at about 52 kDa.
TEAD1 (also called TEF-1) is a TEA-domain transcription factor and one of the main DNA-binding partners of YAP and TAZ in the Hippo pathway. When Hippo signaling is off, YAP/TAZ–TEAD complexes drive genes for proliferation and migration, so TEAD1 is widely studied in organ growth, muscle and heart biology and cancer.
| Target | Transcriptional enhancer factor TEF-1 (gene TEAD1; UniProt P28347, human) |
|---|---|
| Clone | 24T42 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.9 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human SiHa whole cel.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TEAD1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcriptional enhancer factor TEF-1 in the nucleus. Tissue expression noted by UniProt (human): Preferentially expressed in skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenchao Wang et al. (2026) mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via Piezo1-NCOA4-ZBP1 axis. Autophagy. 10.1080/15548627.2026.2677183
