| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tenascin-R; TN-R; Janusin; Restrictin; TNR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Tenascin-R/TNR recombinant protein (Position: E60-R1007). Human TNR shares 94.2% and 93.6% amino acid (aa) sequence identity with mouse and rat TNR, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tenascin-R (gene TNR) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 149.6 kDa, and the supplier reports an observed band at about 180 kDa.
Tenascin-R (TNR) is an extracellular matrix glycoprotein made mainly by oligodendrocytes and some neurons in the central nervous system. It organizes the brain matrix, including perineuronal nets, and can either support adhesion or repel and inhibit neurite growth depending on context, so it is studied in neural development, synaptic plasticity and nerve regeneration.
| Target | Tenascin-R (gene TNR; UniProt Q92752, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 149.6 kDa |
| Observed MW | 180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Tenascin-R/TNR fragment (Glu60–Arg1007), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94.2% and 93.6%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Brain specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
