Anti-TFF2 Rabbit Monoclonal Antibody

SKU:BHA21010094
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Boster Bio
Boster Bio
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Overview
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Anti-TFF2 antibody (Rabbit monoclonal, clone 26T01, IgG). Validated for Western blot. Reactivity: Human. Recognizes trefoil factor 2. Observed band in Western blot: about 16 kDa.
Target TFF2
clone number 26T01
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M07013 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:500-2000<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target TFF2
Alternative names Trefoil factor 2; Spasmolysin; Spasmolytic polypeptide; SP; TFF2; SML1
UniProt # Q03403
Host Rabbit
Clone 26T01
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human TFF2
Molecular weight 14.3 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Secreted.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M07013
Main SKU BHA21010094
Rabbit Monoclonal

Product Overview

Clone 26T01 is a rabbit monoclonal antibody that recognizes trefoil factor 2 (gene TFF2) in human samples and is validated for Western blot. The predicted molecular weight is 14.3 kDa, and the supplier reports an observed band at about 16 kDa.

Trefoil factor 2 (TFF2, also called spasmolytic polypeptide) is a secreted peptide produced by gastric mucous neck cells and Brunner glands. It helps structure the gastric mucus layer, contributes to mucosal protection and repair, and is studied in gastric metaplasia and inflammation.

Target Trefoil factor 2 (gene TFF2; UniProt Q03403, human)
Clone 26T01
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 14.3 kDa
Observed MW 16 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): human stomach.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human TFF2.

Reactivity Notes

The supplier lists reactivity with human. UniProt describes trefoil factor 2 as a secreted protein. Tissue expression noted by UniProt (human): Stomach. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 14.3 kDa; observed band at about 16 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human TFF2.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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