| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI; TFPI1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TFPI |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25T09 is a rabbit monoclonal antibody that recognizes tissue factor pathway inhibitor (gene TFPI) in human samples and is validated for Western blot. The predicted molecular weight is 35.0 kDa, and the supplier reports an observed band at about 35–45 kDa.
Tissue factor pathway inhibitor (TFPI) is a Kunitz-type protease inhibitor that controls the start of blood coagulation. It first binds and inhibits factor Xa and then blocks the tissue factor–factor VIIa complex, and it is studied in hemostasis, thrombosis and hemophilia research.
| Target | Tissue factor pathway inhibitor (gene TFPI; UniProt P10646, human) |
|---|---|
| Clone | 25T09 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.0 kDa |
| Observed MW | 35–45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): U87-MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TFPI.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Mostly in endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
