| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tissue factor pathway inhibitor 2;TFPI-2;Placental protein 5;PP5;TFPI2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TFPI2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tissue factor pathway inhibitor 2 (gene TFPI2) in human and mouse samples and is validated for flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 26.9 kDa; the supplier reports observed bands at 27 kDa, 35 kDa.
Tissue factor pathway inhibitor 2 (TFPI2) is a Kunitz-type serine protease inhibitor secreted into the extracellular matrix. It inhibits plasmin, trypsin and the factor VIIa–tissue factor complex, and it is studied in matrix remodeling, placental biology and as a gene often silenced by methylation in cancers.
| Target | Tissue factor pathway inhibitor 2 (gene TFPI2; UniProt P48307, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 26.9 kDa |
| Observed MW | 27 kDa, 35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): mouse spleen tissue, HeLa cells, human placenta tissue, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): U87 cells, A549 cells.
Immunogen
Synthetic peptide from the N-terminal region of human TFPI2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes tissue factor pathway inhibitor 2 as a secreted protein. Tissue expression noted by UniProt (human): Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guoying Guan et al. (2022) TFPI2 suppresses the interaction of TGF-β2 pathway regulators to promote endothelial–mesenchymal transition in diabetic nephropathy. Journal of Biological Chemistry. 10.1016/j.jbc.2022.101725
