| Field | Specification |
|---|---|
| Target | |
| Alternative names | TGF-beta receptor type-1;TGFR-1;2.7.11.30;Activin A receptor type II-like protein kinase of 53kD;Activin receptor-like kinase 5;ALK-5;ALK5;Serine/threonine-protein kinase receptor R4;SKR4;TGF-beta type I receptor;Transforming growth factor-beta receptor type I;TGF-beta receptor type I;TbetaR-I;TGFBR1;ALK5, SKR4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TGFBR1, identical to the related mouse and rat sequences. |
| Molecular weight | |
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| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody detects TGF-beta receptor type-1 (gene TGFBR1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 56.0 kDa, and the supplier reports an observed band at about 56 kDa.
TGF-β receptor type 1 (TGFBR1, also called ALK5) is a transmembrane serine/threonine kinase. When TGF-β binds the type II receptor, TGFBR1 is recruited and phosphorylated, and it then phosphorylates SMAD2 and SMAD3 to relay signals for growth control, matrix production and immune regulation; it is also a common drug target in fibrosis and cancer studies.
| Target | TGF-beta receptor type-1 (gene TGFBR1; UniProt P36897, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 56.0 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat cardiac muscle tissue, mouse liver tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human TGFBR1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TGF-beta receptor type-1 in the cell membrane, cell junction, cell surface and membrane raft. Tissue expression noted by UniProt (human): Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiao-xu SHEN et al. (2023) Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-β signaling. Journal of Integrative Agriculture. 10.1016/j.jia.2023.05.025
Liu Yixin et al. (2023) CTHRC1 promotes colorectal cancer progression by recruiting tumor-associated macrophages via up-regulation of CCL15. Journal of Molecular Medicine-Jmm. 10.1007/s00109-023-02399-0
Lu Yang et al. (2009) Increased expression of TGFβ type I receptor in brain tissues of patients with temporal lobe epilepsy. Clinical Science. 10.1042/CS20080347
Sha Hong et al. (2018) Abnormal expressions of AGEs, TGF-β1, BDNF and their receptors in diabetic rat colon–Associations with colonic morphometric and biomechanical remodeling. Scientific Reports. 10.1038/s41598-018-27787-2
Ding Rui et al. (2025) Downregulation of ferroptosis-related Genes can regulate the invasion and migration of osteosarcoma cells. Scientific Reports. 10.1038/s41598-025-02319-x
Le-Le Zhang et al. (2018) Nagilactone E suppresses TGF-β1-induced epithelial–mesenchymal transition, migration and invasion in non-small cell lung cancer cells. Phytomedicine. 10.1016/j.phymed.2018.09.222
Jihong Zhan et al. (2019) Oxidative Stress and TGF-β1/Smads Signaling Are Involved in Rosa roxburghii Fruit Extract Alleviating Renal Fibrosis. Evidence-based Complementary and Alternative Medicine. 10.1155/2019/4946580
