| Field | Specification |
|---|---|
| Target | |
| Alternative names | TGF-beta receptor type-1; TGFR-1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5; ALK-5; ALK5; Serine/threonine-protein kinase receptor R4; SKR4; TGF-beta type I receptor; Transforming growth factor-beta receptor type I; TGF-beta receptor type I; TbetaR-I; TGFBR1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TGFBR1 recombinant protein (Position: L34-K490). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets TGF-beta receptor type-1 (gene TGFBR1) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 56.0 kDa, and the supplier reports an observed band at about 56 kDa.
TGF-β receptor type 1 (TGFBR1, also called ALK5) is a transmembrane serine/threonine kinase. When TGF-β binds the type II receptor, TGFBR1 is recruited and phosphorylated, and it then phosphorylates SMAD2 and SMAD3 to relay signals for growth control, matrix production and immune regulation; it is also a common drug target in fibrosis and cancer studies.
| Target | TGF-beta receptor type-1 (gene TGFBR1; UniProt P36897, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 56.0 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TGFBR1 fragment (Leu34–Lys490), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places TGF-beta receptor type-1 in the cell membrane, cell junction, cell surface and membrane raft. Tissue expression noted by UniProt (human): Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhi-Min Geng et al. (2008) Role of transforming growth factor-beta signaling pathway in pathogenesis of benign biliary stricture. World Journal of Gastroenterology. 10.3748/wjg.14.4949
