| Field | Specification |
|---|---|
| Target | |
| Alternative names | TGF-beta receptor type-2;TGFR-2;2.7.11.30;TGF-beta type II receptor;Transforming growth factor-beta receptor type II;TGF-beta receptor type II;TbetaR-II;TGFBR2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TGF beta Receptor II, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets TGF-beta receptor type-2 (gene TGFBR2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 64.6 kDa, and the supplier reports an observed band at about 65 kDa.
TGF-β receptor type 2 (TGFBR2) is a constitutively active transmembrane serine/threonine kinase that binds TGF-β1, β2 and β3 directly. Ligand binding lets it recruit and phosphorylate TGFBR1, launching SMAD signaling, and TGFBR2 mutations are linked to Loeys-Dietz syndrome and to some cancers.
| Target | TGF-beta receptor type-2 (gene TGFBR2; UniProt P37173, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 64.6 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): HepG2 cells, COLO320 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the middle region of human TGF beta Receptor II. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TGF-beta receptor type-2 in the cell membrane, membrane raft and SUBCELLULAR LOCATION:. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Le-Le Zhang et al. (2018) Nagilactone E suppresses TGF-β1-induced epithelial–mesenchymal transition, migration and invasion in non-small cell lung cancer cells. Phytomedicine. 10.1016/j.phymed.2018.09.222
Zhouguang Wu et al. (2024) Hsa_circ_0009096/miR-370-3p modulates hepatic stellate cell proliferation and fibrosis during biliary atresia pathogenesis. PeerJ. 10.7717/peerj.17356
Hong Sha et al. (2015) Mechanism Investigation of the Improvement of Chang Run Tong on the Colonic Remodeling in Streptozotocin-Induced Diabetic Rats. Journal of Diabetes Research. 10.1155/2016/1826281
