| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transforming growth factor beta receptor type 3; TGF-beta receptor type 3; TGFR-3; Betaglycan; Transforming growth factor beta receptor III; TGF-beta receptor type III; TGFBR3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TGF beta Receptor III/TGFBR3 recombinant protein (Position: H37-R649). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transforming growth factor beta receptor type 3 (gene TGFBR3) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 93.5 kDa, and the supplier reports an observed band at about 150 kDa.
TGF-β receptor type 3 (TGFBR3, also called betaglycan) is a proteoglycan co-receptor with no kinase activity. It binds TGF-β isoforms, inhibin and some BMPs and presents them to signaling receptors, notably enhancing TGF-β2 signaling, and it is studied in development and cancer.
| Target | Transforming growth factor beta receptor type 3 (gene TGFBR3; UniProt Q03167, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 93.5 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Recombinant human TGF beta Receptor III/TGFBR3 fragment (His37–Arg649), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes transforming growth factor beta receptor type 3 as secreted and also located in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
