| Field | Specification |
|---|---|
| Target | |
| Alternative names | TGF-beta receptor type-2; TGFR-2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II; TGFBR2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TGFBR2, different from the related mouse sequence by five amino acids, and from the related rat sequence by eight amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 2F11 is a mouse monoclonal antibody that recognizes TGF-beta receptor type-2 (gene TGFBR2) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 64.6 kDa, and the supplier reports an observed band at about 70–85 kDa.
TGF-β receptor type 2 (TGFBR2) is a constitutively active transmembrane serine/threonine kinase that binds TGF-β1, β2 and β3 directly. Ligand binding lets it recruit and phosphorylate TGFBR1, launching SMAD signaling, and TGFBR2 mutations are linked to Loeys-Dietz syndrome and to some cancers.
| Target | TGF-beta receptor type-2 (gene TGFBR2; UniProt P37173, human) |
|---|---|
| Clone | 2F11 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 64.6 kDa |
| Observed MW | 70–85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human A549 cells, human K562 cells, human Caco-2 cells, human HeLa cells, human T-47D cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue, human cervical intraepithelial neoplasia tissue, human esophageal squamous carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HepG2 cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the N-terminal region of human TGFBR2. It differs from the mouse sequence at five residues and from the rat sequence at eight residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places TGF-beta receptor type-2 in the cell membrane, membrane raft and SUBCELLULAR LOCATION:. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hao Li et al. (2015) TGF-β Induces Degradation of PTHrP Through Ubiquitin-Proteasome System in Hepatocellular Carcinoma. Journal of Cancer. 10.7150/jca.10830
Fang Weiyi et al. (2008) Transcriptional patterns, biomarkers and pathways characterizing nasopharyngeal carcinoma of Southern China. Journal of Translational Medicine. 10.1186/1479-5876-6-32
