| Field | Specification |
|---|---|
| Target | |
| Alternative names | Acyl-coenzyme A thioesterase THEM4; Acyl-CoA thioesterase THEM4; Carboxyl-terminal modulator protein; Thioesterase superfamily member 4; THEM4; CTMP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human THEM4 recombinant protein (Position: M1-T240). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets acyl-coenzyme A thioesterase THEM4 in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 27.1 kDa, and the supplier reports an observed band at about 22 kDa.
THEM4 (also called CTMP) is an acyl-CoA thioesterase that hydrolyzes medium- and long-chain fatty acyl-CoAs and is thought to take part in mitochondrial fatty acid metabolism. It has also been reported to bind AKT1 and modulate its activity, linking it to apoptosis control. THEM4 is studied in lipid metabolism, mitochondrial function and AKT signaling.
| Target | Acyl-coenzyme A thioesterase THEM4 (gene THEM4; UniProt Q5T1C6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 27.1 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): PC-3 cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human THEM4 fragment (Met1–Thr240), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places acyl-coenzyme A thioesterase THEM4 in the cell membrane, cell projection, cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Expressed predominantly in skeletal muscle, testis, uterus, brain and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
