| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thioredoxin, mitochondrial; MTRX; Mt-Trx; Thioredoxin-2; TXN2; TRX2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Thioredoxin 2/TXN2 recombinant protein (Position: T60-G166). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 4H3 is a mouse monoclonal antibody that recognizes thioredoxin, mitochondrial (gene TXN2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 18.4 kDa, and the supplier reports an observed band at about 14 kDa.
Thioredoxin-2 (TXN2) is the mitochondrial form of thioredoxin, a small protein that reduces disulfide bonds through its active-site cysteines. Supported by thioredoxin reductase 2, it helps control mitochondrial reactive oxygen species and plays a role in apoptosis regulation and cell survival.
| Target | Thioredoxin, mitochondrial (gene TXN2; UniProt Q99757, human) |
|---|---|
| Clone | 4H3 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 18.4 kDa |
| Observed MW | 14 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human K562 cells, human A549 cells, human Caco-2 cells, human THP-1 cells, rat PC-12 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human gastric cancer tissue, human lung cancer tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells, MCF-7 cells.
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human Thioredoxin 2/TXN2 fragment (Thr60–Gly166), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places thioredoxin, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Widely expressed in adult (at protein level) and fetal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
