| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proteinase-activated receptor 1;PAR-1;Coagulation factor II receptor;Thrombin receptor;F2R;CF2R, PAR1, TR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Thrombin Receptor. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects proteinase-activated receptor 1 (gene F2R) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 47.4 kDa, and the supplier reports an observed band at about 47 kDa.
Proteinase-activated receptor 1 (PAR1, encoded by F2R) is the main thrombin receptor. Thrombin cleaves its N-terminus to expose a tethered ligand that activates the G protein-coupled receptor, triggering calcium signaling and platelet activation. PAR1 also acts on endothelial and other cells and is a target of antiplatelet drugs.
| Target | Proteinase-activated receptor 1 (gene F2R; UniProt P25116, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 47.4 kDa |
| Observed MW | 47 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): MCF-7 cells, HeLa cells, 22RV1 cells, SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of human thrombin Receptor.
Reactivity Notes
The supplier lists reactivity with human. UniProt places proteinase-activated receptor 1 in the cell membrane. Tissue expression noted by UniProt (human): Platelets and vascular endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
