| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thrombospondin-1;THBS1;TSP, TSP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human THBS1, different from the related mouse sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects thrombospondin-1 (gene THBS1) in human samples and is validated for Western blot. The predicted molecular weight is 129.4 kDa, and the supplier reports an observed band at about 160 kDa.
Thrombospondin-1 (THBS1, TSP-1) is a large matricellular glycoprotein released from platelets and many other cells that mediates cell–cell and cell–matrix interactions. Acting through receptors such as CD36 and CD47, it inhibits angiogenesis, dampens nitric oxide signaling and helps activate latent TGF-β. TSP-1 is studied in wound healing, vascular biology, inflammation and cancer.
| Target | Thrombospondin-1 (gene THBS1; UniProt P07996, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 129.4 kDa |
| Observed MW | 160 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HepG2 cells, human K562 cells, rat C6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human THBS1. It differs from the mouse sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes thrombospondin-1 as secreted and also located in the cell surface, endoplasmic reticulum and sarcoplasmic reticulum. Tissue expression noted by UniProt (human): Expressed by platelets (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fan Xiaorong et al. (2021) Differential expression of microRNAs in human endometrium after implantation of an intrauterine contraceptive device containing copper. Molecular Human Reproduction. 10.1093/molehr/gaab052
Fan Xiao-rong et al. (2025) The Regulatory Mechanism of MicroRNA-144-3p on Damage to Endometrial Epithelial Cells Exposed to Copper Ions In Vitro. Current Medical Science. 10.1007/s11596-025-00110-7
