| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thymidylate synthase; TS; TSase; TYMS |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Thymidylate Synthase Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADAA-20 is a rabbit monoclonal antibody that recognizes thymidylate synthase (gene TYMS) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 35.7 kDa, and the supplier reports an observed band at about 36 kDa.
Thymidylate synthase (TYMS) converts dUMP to dTMP using methylenetetrahydrofolate as the methyl donor, providing the only de novo source of thymidine nucleotides for DNA synthesis. Because dividing cells depend on this reaction, TYMS is the target of chemotherapy drugs such as 5-fluorouracil and pemetrexed.
| Target | Thymidylate synthase (gene TYMS; UniProt P04818, human) |
|---|---|
| Clone | ADAA-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.7 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human 293T cells, monkey COS-7 cells, rat thymus tissue, rat PC-12 cells, mouse thymus tissue, mouse SP2/0 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human B lymphoblastoma tissue, human spleen tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human thymidylate Synthase Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places thymidylate synthase in the nucleus, cytoplasm, mitochondrion and mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Peng Yan et al. (2025) Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence. OncoTargets and Therapy. 10.2147/OTT.S508577
Yan Huang et al. (2014) Predictive value of thymidylate synthase for the prognosis and survival of lung adenocarcinoma patients. Oncology Letters. 10.3892/ol.2014.2658
