| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thyroglobulin; Tg |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Thyroglobulin Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) . |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFEC-20 is a rabbit monoclonal antibody that recognizes thyroglobulin (gene TG) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 304.8 kDa, and the supplier reports an observed band at about 305 kDa.
Thyroglobulin (TG) is a very large glycoprotein made by thyroid follicular cells and stored in the follicle lumen. Selected tyrosines on thyroglobulin are iodinated and coupled to form the thyroid hormones T4 and T3, which are released after thyroglobulin is taken back up and degraded; serum thyroglobulin is also a standard marker in thyroid cancer follow-up.
| Target | Thyroglobulin (gene TG; UniProt P01266, human) |
|---|---|
| Clone | AFEC-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 304.8 kDa |
| Observed MW | 305 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): mouse thyroid.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human thyroid cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human thyroglobulin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes thyroglobulin as a secreted protein. Tissue expression noted by UniProt (human): Specifically expressed in the thyroid gland. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
