| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-1 receptor;2.7.10.1;Endothelial tyrosine kinase;Tunica interna endothelial cell kinase;Tyrosine kinase with Ig and EGF homology domains-2;Tyrosine-protein kinase receptor TEK;Tyrosine-protein kinase receptor TIE-2;hTIE2;p140 TEK;CD202b;TEK;TIE2, VMCM, VMCM1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TIE2 recombinant protein (Position: Q641-I830). Human TIE2 shares 91% amino acid (aa) sequence identity with mouse TIE2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes angiopoietin-1 receptor (gene TEK) in human samples and is validated for Western blot. The predicted molecular weight is 125.8 kDa, and the supplier reports an observed band at about 125 kDa.
TIE2 (TEK) is a receptor tyrosine kinase on endothelial cells that binds angiopoietin-1 and angiopoietin-2. Angiopoietin-1 signaling through TIE2 promotes endothelial survival and vascular stability, while angiopoietin-2 can oppose it, making TIE2 a central subject in angiogenesis, vascular leak and lymphatic development research.
| Target | Angiopoietin-1 receptor (gene TEK; UniProt Q02763, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 125.8 kDa |
| Observed MW | 125 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human TIE2 fragment (Gln641–Ile830), expressed in E. coli. Sequence identity with the mouse ortholog: 91%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes angiopoietin-1 receptor as secreted and also located in the cell membrane, cell junction and cytoplasm. Tissue expression noted by UniProt (human): Detected in umbilical vein endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
