| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-1 receptor; Endothelial tyrosine kinase; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; hTIE2; p140 TEK; CD202b; TEK; TIE2; VMCM; VMCM1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TIE2/TEK, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects angiopoietin-1 receptor (gene TEK) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 125.8 kDa, and the supplier reports an observed band at about 160 kDa.
TIE2 (TEK) is a receptor tyrosine kinase on endothelial cells that binds angiopoietin-1 and angiopoietin-2. Angiopoietin-1 signaling through TIE2 promotes endothelial survival and vascular stability, while angiopoietin-2 can oppose it, making TIE2 a central subject in angiogenesis, vascular leak and lymphatic development research.
| Target | Angiopoietin-1 receptor (gene TEK; UniProt Q02763, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 125.8 kDa |
| Observed MW | 160 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human Caco-2 cells, human HeLa cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human mammary cancer tissue, human placenta tissue, mouse heart tissue, mouse lung tissue, rat heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the N-terminal region of human TIE2/TEK. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes angiopoietin-1 receptor as secreted and also located in the cell membrane, cell junction and cytoplasm. Tissue expression noted by UniProt (human): Detected in umbilical vein endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lu Liu et al. (2017) Transplantation of Human Umbilical Cord Blood Mononuclear Cells Attenuated Ischemic Injury in MCAO Rats via Inhibition of NF-κB and NLRP3 Inflammasome. Neuroscience. 10.1016/j.neuroscience.2017.11.027
Yan Wu et al. (2023) Saikosaponin-d regulates angiogenesis in idiopathic pulmonary fibrosis through angiopoietin/Tie-2 pathway. ACTA Histochemica. 10.1016/j.acthis.2023.152100
Hua Yang et al. (2010) Eukaryotic expression of extracellular ligand binding domains of murine Tie-2 and its anti-angiogenesis effect in SGC-7901 cell lines. Journal of Gastroenterology and Hepatology. 10.1111/j.1440-1746.2009.05997.x
Qingping Zheng et al. (2011) Exercise Improves Recovery after Ischemic Brain Injury by Inducing the Expression of Angiopoietin-1 and Tie-2 in Rats. Tohoku Journal of Experimental Medicine. 10.1620/tjem.224.221
X. Ma et al. (2008) Expressions of Angiopoietin-1, Angiopoietin-2, and Tie2 and Their Roles in Rat Renal Allografts With Chronic Allograft Nephropathy. Transplantation Proceedings. 10.1016/j.transproceed.2008.07.121
X. Ma et al. (2008) Rapamycin and Cyclosporine Have Different Effects on Expression of ang-1 and ang-2 and Tie2 in Rat Renal Allograft With Chronic Allograft Nephropathy. Transplantation Proceedings. 10.1016/j.transproceed.2008.08.047
