| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial import inner membrane translocase subunit TIM44; TIMM44; MIMT44; TIM44 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TIM44 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30T70 is a rabbit monoclonal antibody that recognizes mitochondrial import inner membrane translocase subunit TIM44 (gene TIMM44) in human and rat samples and is validated for Western blot, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 51.4 kDa, and the supplier reports an observed band at about 45 kDa.
TIM44 (TIMM44) is a peripheral inner membrane protein and key part of the import motor that drives proteins into the mitochondrial matrix. It anchors mitochondrial HSP70 at the TIM23 channel so that ATP hydrolysis can pull incoming precursor proteins inward. TIM44 is studied in mitochondrial protein import and biogenesis.
| Target | Mitochondrial import inner membrane translocase subunit TIM44 (gene TIMM44; UniProt O43615, human) |
|---|---|
| Clone | 30T70 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.4 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): K562 cells, C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TIM44.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places mitochondrial import inner membrane translocase subunit TIM44 in the mitochondrion inner membrane and mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
