| Field | Specification |
|---|---|
| Target | |
| Alternative names | Metalloproteinase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1; TIMP1; CLGI, TIMP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse TIMP1 recombinant protein (Position: H31-N193). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets metalloproteinase inhibitor 1 (gene Timp1) in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 22.6 kDa, and the supplier reports an observed band at about 28 kDa.
TIMP-1 is a secreted tissue inhibitor of metalloproteinases that binds the catalytic zinc site of many matrix metalloproteinases, including MMP-9, and blocks their activity. It also has growth factor–like, MMP-independent effects on cell survival and proliferation. TIMP-1 is studied in extracellular matrix remodeling, fibrosis, inflammation and cancer.
| Target | Metalloproteinase inhibitor 1 (gene Timp1; UniProt P12032, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 22.6 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse lung tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): RAW264.7 cells, RH35 cells.
Immunogen
Recombinant mouse TIMP1 fragment (His31–Asn193), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes metalloproteinase inhibitor 1 as a secreted protein. Tissue expression noted by UniProt (mouse): Found in fetal and adult tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ping-Fang Hu et al. (2009) Adenovirus-mediated transfer of siRNA against PAI-1 mRNA ameliorates hepatic fibrosis in rats. Journal of Hepatology. 10.1016/j.jhep.2009.02.025
Guan-Ying Han et al. (2014) Xanthan gum inhibits cartilage degradation by down-regulating matrix metalloproteinase-1 and -3 expressions in experimental osteoarthritis. Journal of Bioactive and Compatible Polymers. 10.1177/0883911514521916
Huang Jiaquan et al. (2009) Effect of anluohuaxian tablet combined with γ-IFN on schistosomal liver fibrosis. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-009-0111-7
