| Field | Specification |
|---|---|
| Target | |
| Alternative names | Metalloproteinase inhibitor 2;CSC-21K;Tissue inhibitor of metalloproteinases 2;TIMP-2;TIMP2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human TIMP2 recombinant protein (Position: C27-P220). Human TIMP2 shares 98.5% amino acid (aa) sequence identity with both mouse and rat TIMP2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes metalloproteinase inhibitor 2 (gene TIMP2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 24.4 kDa, and the supplier reports an observed band at about 24 kDa.
TIMP-2 is a secreted tissue inhibitor of metalloproteinases that inhibits a broad range of MMPs. It also has a special role in activating pro-MMP-2 at the cell surface together with MT1-MMP (MMP-14). TIMP-2 is studied in extracellular matrix turnover, angiogenesis, kidney injury and tumor biology.
| Target | Metalloproteinase inhibitor 2 (gene TIMP2; UniProt P16035, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 24.4 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat spleen tissue, rat testis tissue, mouse brain tissue, mouse thymus tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TIMP2 fragment (Cys27–Pro220), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes metalloproteinase inhibitor 2 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ling Fang et al. (2014) TGF-β1-elevated TRPM7 channel regulates collagen expression in hepatic stellate cells via TGF-β1/Smad pathway. Toxicology and Applied Pharmacology. 10.1016/j.taap.2014.08.006
Cheng Gao et al. (2009) IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation. Cellular and Molecular Neurobiology. 10.1007/s10571-009-9409-z
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Ye Song et al. (2003) Fluvastatin prevents nephropathy likely through suppression of connective tissue growth factor-mediated extracellular matrix accumulation. Experimental and Molecular Pathology. 10.1016/j.yexmp.2003.08.002
Yunshan Chen et al. (2023) Trophoblast cellular adhesion molecule expression regulated by different genes and their correlation with pregnancy-induced hypertension. Cellular and Molecular Biology. 10.14715/cmb/2023.69.12.5
Xi Zheng et al. (2014) Intermittent cold stress enhances features of atherosclerotic plaque instability in apolipoprotein E‑deficient mice. Molecular Medicine Reports. 10.3892/mmr.2014.2464
Cheng-Cheng Yang et al. (2023) 17β-estradiol inhibits TGF-β-induced collagen gel contraction mediated by human Tenon fibroblasts via Smads and MAPK signaling pathways. International Journal of Ophthalmology. 10.18240/ijo.2023.09.10
Yan Zhou et al. (2016) Effects of electromagnetic pulse exposure on gelatinase of blood–brain barrier in vitro. Electromagnetic Biology and Medicine. 10.3109/15368378.2016.1140058
Long Junrong et al. (2019) H2S attenuates the myocardial fibrosis in diabetic rats through modulating PKC-ERK1/2MAPK signaling pathway. Technology and Health Care. 10.3233/THC-199029
