| Field | Specification |
|---|---|
| Target | |
| Alternative names | Metalloproteinase inhibitor 2;CSC-21K;Tissue inhibitor of metalloproteinases 2;TIMP-2;TIMP2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TIMP2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AADE-20 is a rabbit monoclonal antibody that recognizes metalloproteinase inhibitor 2 (gene TIMP2) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 24.4 kDa, and the supplier reports an observed band at about 22 kDa.
TIMP-2 is a secreted tissue inhibitor of metalloproteinases that inhibits a broad range of MMPs. It also has a special role in activating pro-MMP-2 at the cell surface together with MT1-MMP (MMP-14). TIMP-2 is studied in extracellular matrix turnover, angiogenesis, kidney injury and tumor biology.
| Target | Metalloproteinase inhibitor 2 (gene TIMP2; UniProt P16035, human) |
|---|---|
| Clone | AADE-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.4 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TIMP2.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes metalloproteinase inhibitor 2 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei-Jiang Zhao et al. (2022) LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion. Journal of Cancer. 10.7150/jca.71256
