| Field | Specification |
|---|---|
| Target | |
| Alternative names | Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human TIMP3 recombinant protein (Position: C24-P211). Human TIMP3 shares 97.9% and 97.3% amino acid (aa) sequence identity with mouse and rat TIMP3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects metalloproteinase inhibitor 3 (gene TIMP3) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 24.1 kDa, and the supplier reports an observed band at about 34 kDa.
TIMP-3 is a tissue inhibitor of metalloproteinases that binds to the extracellular matrix rather than circulating freely. Besides MMPs, it inhibits several ADAM and ADAMTS proteases, including the TNF-α-converting enzyme ADAM17, affecting matrix turnover, inflammation and angiogenesis. Mutations in TIMP3 cause Sorsby fundus dystrophy, a retinal degeneration.
| Target | Metalloproteinase inhibitor 3 (gene TIMP3; UniProt P35625, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 24.1 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): rat kidney tissue, mouse ovary tissue, HeLa cells, MCF-7 cells, SMMC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TIMP3 fragment (Cys24–Pro211), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 97.9% and 97.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chihao Lin et al. (2026) Platelet-rich plasma cues program M2 macrophage-derived exosome mimetics to regenerate the tendon-bone interface. Journal of Nanobiotechnology. 10.1186/s12951-026-04626-5
Jing Wen et al. (2025) GC-MSCs transcriptionally upregulate SALL4 in gastric cancer through miR-4669/TIMP3/β-catenin signaling. Cellular Signalling. 10.1016/j.cellsig.2025.111668
Liangui Nie et al. (2021) Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway. Molecular Medicine Reports. 10.3892/mmr.2021.11938
Guan ZhiYu et al. (2013) Promoter methylation and expression of TIMP3 gene in gastric cancer. Diagnostic Pathology. 10.1186/1746-1596-8-110
Yu-Xian Bai et al. (2007) Clinicopathologic significance of BAG1 and TIMP3 expression in colon carcinoma. World Journal of Gastroenterology. 10.3748/wjg.v13.i28.3883
