| Field | Specification |
|---|---|
| Target | |
| Alternative names | Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TIMP3, different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes metalloproteinase inhibitor 3 (gene TIMP3) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 24.1 kDa, and the supplier reports an observed band at about 24 kDa.
TIMP-3 is a tissue inhibitor of metalloproteinases that binds to the extracellular matrix rather than circulating freely. Besides MMPs, it inhibits several ADAM and ADAMTS proteases, including the TNF-α-converting enzyme ADAM17, affecting matrix turnover, inflammation and angiogenesis. Mutations in TIMP3 cause Sorsby fundus dystrophy, a retinal degeneration.
| Target | Metalloproteinase inhibitor 3 (gene TIMP3; UniProt P35625, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 24.1 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
Samples with a confirmed band (WB): rat kidney tissue, NIH3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human TIMP3. It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shiwen Liu et al. (2021) Endosulfan promotes cell proliferation and extracellular matrix accumulation through TGF-β/Smad signaling pathway in HRMCs. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2021.113040
