| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tight junction protein 2; Tight junction protein ZO-2; Zona occludens protein 2; Zonula occludens protein 2; TJP2; X104; ZO2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at C-terminus of human TJP2/ZO2, identical to the related mouse sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tight junction protein 2 (gene TJP2) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 134.0 kDa, and the supplier reports an observed band at about 150 kDa.
ZO-2 (TJP2) is a MAGUK scaffolding protein that, together with ZO-1 and ZO-3, links tight junction membrane proteins to the actin cytoskeleton. It is found at tight and adherens junctions and can also shuttle to the nucleus. TJP2 mutations cause a form of familial hypercholanemia and progressive cholestasis, and it is studied in epithelial barrier and liver biology.
| Target | Tight junction protein 2 (gene TJP2; UniProt Q9UDY2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 134.0 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Hek293 cells, monkey COS-7 cells, human HeLa cells, human MCF-7 cells, human HepG2 cells, rat brain tissue, mouse brain tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human ovarian serous adenocarcinoma tissue, human rectal cancer tissue, human ovarian cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Synthetic peptide based on the sequence of at C-terminus of human TJP2/ZO2. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places tight junction protein 2 in the cell junction, cell membrane and nucleus. Tissue expression noted by UniProt (human): This protein is found in epithelial cell junctions. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
