| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transketolase-like protein 1; Transketolase 2; TK 2; Transketolase-related protein; TKTL1; TKR; TKT2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TKR/TKTL1, which shares 68.2% amino acid (aa) sequence identity with mouse TKTL1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transketolase-like protein 1 (gene TKTL1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 65.3 kDa, and the supplier reports an observed band at about 65 kDa.
TKTL1 (transketolase-like protein 1) is a homolog of transketolase, the thiamine-dependent enzyme of the non-oxidative pentose phosphate pathway. It is predicted to transfer two-carbon ketol groups between sugar phosphates, although its enzymatic activity in cells remains debated. TKTL1 has been studied in cancer metabolism and, more recently, in neocortex development.
| Target | Transketolase-like protein 1 (gene TKTL1; UniProt P51854, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 65.3 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human MOLT-4 cells, rat testis tissue, rat PC-12 cells, mouse testis tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human TKR/TKTL1. Sequence identity with the mouse ortholog: 68.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transketolase-like protein 1 in the cytoplasm. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
