| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 2;Toll/interleukin-1 receptor-like protein 4;CD282;TLR2;TIL4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TLR2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADH-20 is a rabbit monoclonal antibody that recognizes toll-like receptor 2 (gene TLR2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 89.8 kDa, and the supplier reports an observed band at about 90 kDa.
Toll-like receptor 2 (TLR2) is a cell surface pattern recognition receptor that pairs with TLR1 or TLR6 to recognize bacterial lipoproteins, lipopeptides and other cell wall components. Through MyD88 and TRAF6 it activates NF-κB and triggers inflammatory cytokine release. TLR2 is widely studied in innate immunity, bacterial and fungal infection, and chronic inflammation.
| Target | Toll-like receptor 2 (gene TLR2; UniProt O60603, human) |
|---|---|
| Clone | ADH-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 89.8 kDa |
| Observed MW | 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TLR2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places toll-like receptor 2 in the membrane, cytoplasmic vesicle and membrane raft. Tissue expression noted by UniProt (human): Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Ying et al. (2014) Protection of ischemic post conditioning against transient focal ischemia-induced brain damage is associated with inhibition of neuroinflammation via modulation of TLR2 and TLR4 pathways. Journal of Neuroinflammation. 10.1186/1742-2094-11-15
Suoyi Feng et al. (2022) Therapeutic Effect of C-C Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis. Journal of Inflammation Research. 10.2147/JIR.S254717
Peng Jialing et al. (2016) Up-regulated TLR2 and TLR4 expressions in liver and spleen during acute murine T. gondii infection. Parasitology Research. 10.1007/s00436-016-5226-8
ANNA K. GERGEN et al. (2020) Activation of Toll-Like Receptor 2 Promotes Proliferation of Human Lung Adenocarcinoma Cells. Anticancer Research. 10.21873/anticanres.14544
Xiaohui Jin et al. (2020) Porcine parvovirus nonstructural protein NS1 activates NF-κB and it involves TLR2 signaling pathway. Journal of Veterinary Science. 10.4142/jvs.2020.21.e50
