| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 4; hToll; CD antigen CD284; TLR4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TLR4 recombinant protein (Position: Q39-Q616). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects toll-like receptor 4 (gene TLR4) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 95.7 kDa, and the supplier reports an observed band at about 100 kDa.
Toll-like receptor 4 (TLR4) is the receptor for bacterial lipopolysaccharide (LPS), working with the co-receptor MD-2 (LY96) and CD14. It signals from the cell surface through MyD88 and, after internalization, through TRIF, activating NF-κB, IRF3 and inflammatory cytokine production. TLR4 is central to research on sepsis, endotoxin responses and sterile inflammation.
| Target | Toll-like receptor 4 (gene TLR4; UniProt O00206, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 95.7 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human hcct tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human TLR4 fragment (Gln39–Gln616), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places toll-like receptor 4 in the cell membrane, early endosome and cell projection. Tissue expression noted by UniProt (human): Highly expressed in placenta, spleen and peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yao Xu et al. (2018) Discovery of novel small molecule TLR4 inhibitors as potent anti-inflammatory agents. European Journal of Medicinal Chemistry. 10.1016/j.ejmech.2018.05.033
Huaping Chen et al. (2019) Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells. Mediators of Inflammation. 10.1155/2019/7538071
