| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 5;Toll/interleukin-1 receptor-like protein 3;TLR5;TIL3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TLR5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AADF-20 is a rabbit monoclonal antibody that recognizes toll-like receptor 5 (gene TLR5) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 97.8 kDa, and the supplier reports an observed band at about 97 kDa.
Toll-like receptor 5 (TLR5) is a cell surface pattern recognition receptor that recognizes flagellin, the main protein of bacterial flagella. It signals through MyD88 to activate NF-κB and inflammatory gene expression and is prominent on intestinal epithelial and immune cells. TLR5 is studied in mucosal immunity, gut microbiota and bacterial infection.
| Target | Toll-like receptor 5 (gene TLR5; UniProt O60602, human) |
|---|---|
| Clone | AADF-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 97.8 kDa |
| Observed MW | 97 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human TLR5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places toll-like receptor 5 in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed on the basolateral surface of intestinal epithelia.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
