Anti-TLR5 Rabbit Monoclonal Antibody

Overview

Anti-TLR5 Rabbit Monoclonal Antibody is an antibody targeting TLR5. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AADF-20; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 73 kDa; calculated MW: 97834 MW.

Boster Bio Anti-TLR5 Rabbit Monoclonal Antibody catalog # M00462. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: TLR5 — Toll-like receptor 5
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AADF-20; Isotype: Rabbit IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 73 kDa; Calculated: 97834 MW

Biological background

Protein function (datasheet): Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. .

Cellular localization (datasheet): Membrane ; Single-pass type I membrane protein .

Tissue details (datasheet): Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.

Research relevance and current trends

• Commonly studied in contexts related to Immunoglobulins,Immunology,Innate Immunity,Macrophage/Inflammation,Receptors,TLR Signaling.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.