| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Toll-like receptor 7;TLR7;UNQ248/PRO285; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human TLR7 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TLR7 Rabbit Monoclonal Antibody is an antibody targeting TLR7. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ABOO-20; isotype: Rabbit IgG; reactivity: Human; observed MW: 125 kDa; calculated MW: 120922 MW.
Boster Bio Anti-TLR7 Rabbit Monoclonal Antibody catalog # M00376. Tested in WB, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: TLR7 — Toll-like receptor 7
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ABOO-20; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 125 kDa; Calculated: 120922 MW
Biological background
Protein function (datasheet): Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). .
Cellular localization (datasheet): Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Endosome . Lysosome . Cytoplasmic vesicle, phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. .
Tissue details (datasheet): Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
Research relevance and current trends
- Commonly studied in contexts related to Transcription,Transmembrane.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
