| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 8; CD antigen CD288; TLR8 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at N-terminus of human TLR8, which shares 76.5% amino acid (aa) sequence identity with mouse TLR8. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects toll-like receptor 8 (gene TLR8) in human samples and is validated for IHC. The predicted molecular weight is 119.8 kDa.
Toll-like receptor 8 (TLR8) is an endosomal pattern recognition receptor that recognizes fragments of single-stranded RNA, particularly GU-rich sequences from viruses and bacteria. It signals through MyD88 to activate NF-κB and pro-inflammatory cytokines, mainly in monocytes and myeloid dendritic cells. TLR8 is studied in antiviral immunity and as a target for vaccine adjuvants.
| Target | Toll-like receptor 8 (gene TLR8; UniProt Q9NR97, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 119.8 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|
Samples with confirmed staining (IHC): human gastric cancer tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of at N-terminus of human TLR8. Sequence identity with the mouse ortholog: 76.5%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places toll-like receptor 8 in the endosome membrane. Tissue expression noted by UniProt (human): Expressed in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
