| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 8; CD antigen CD288; Tlr8 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse Tlr8, which shares 76.5% amino acid (aa) sequence identity with human TLR8. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects toll-like receptor 8 (gene Tlr8) in mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 119.4 kDa, and the supplier reports an observed band at about 119 kDa.
Toll-like receptor 8 (TLR8) is an endosomal pattern recognition receptor that recognizes fragments of single-stranded RNA, particularly GU-rich sequences from viruses and bacteria. It signals through MyD88 to activate NF-κB and pro-inflammatory cytokines, mainly in monocytes and myeloid dendritic cells. TLR8 is studied in antiviral immunity and as a target for vaccine adjuvants.
| Target | Toll-like receptor 8 (gene Tlr8; UniProt P58682, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 119.4 kDa |
| Observed MW | 119 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): mouse Ana-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of mouse tlr8. Sequence identity with the human ortholog: 76.5%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places toll-like receptor 8 in the endosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhao Lin-Xia et al. (2020) TLR8 in the Trigeminal Ganglion Contributes to the Maintenance of Trigeminal Neuropathic Pain in Mice. Neuroscience Bulletin. 10.1007/s12264-020-00621-4
Jianzhong Han et al. (2013) Inhibition of lipopolysaccharide-mediated rat alveolar macrophage activation in vitro by antiflammin-1. Cell Biology International. 10.1016/j.cellbi.2008.04.023
