| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 9; CD antigen CD289; TLR9 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TLR9, which shares 97.1% amino acid (aa) sequence identity with mouse TLR9. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets toll-like receptor 9 (gene TLR9) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 115.9 kDa, and the supplier reports an observed band at about 116 kDa.
Toll-like receptor 9 (TLR9) is an endosomal pattern recognition receptor that detects unmethylated CpG motifs in bacterial and viral DNA. It signals through MyD88 to activate NF-κB and IRF7, leading to cytokine and type I interferon production, especially in plasmacytoid dendritic cells and B cells. TLR9 is studied in antimicrobial immunity, autoimmunity and CpG-based adjuvants.
| Target | Toll-like receptor 9 (gene TLR9; UniProt Q9NR96, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 115.9 kDa |
| Observed MW | 116 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human Raji cells, human Daudi cells, human MOLT-4 cells, rat thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human TLR9. Sequence identity with the mouse ortholog: 97.1%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places toll-like receptor 9 in the endoplasmic reticulum membrane, early endosome membrane, lysosome and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
