| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 9;CD289;TLR9;UNQ5798/PRO19605; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TLR9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABFH-20 is a rabbit monoclonal antibody that recognizes toll-like receptor 9 (gene TLR9) in human samples and is validated for Western blot. The predicted molecular weight is 115.9 kDa, and the supplier reports an observed band at about 130 kDa.
Toll-like receptor 9 (TLR9) is an endosomal pattern recognition receptor that detects unmethylated CpG motifs in bacterial and viral DNA. It signals through MyD88 to activate NF-κB and IRF7, leading to cytokine and type I interferon production, especially in plasmacytoid dendritic cells and B cells. TLR9 is studied in antimicrobial immunity, autoimmunity and CpG-based adjuvants.
| Target | Toll-like receptor 9 (gene TLR9; UniProt Q9NR96, human) |
|---|---|
| Clone | ABFH-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 115.9 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TLR9.
Reactivity Notes
The supplier lists reactivity with human. UniProt places toll-like receptor 9 in the endoplasmic reticulum membrane, early endosome membrane, lysosome and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
